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divIVA [2017-07-05 12:49:32]
curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septum, cell-division initiation protein (septum placement), part of the Min system (with Z ring placement)
Molecular weight
19.20 kDa
Product
cell-division initiation protein
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,612,521 → 1,613,015
Phenotypes of a mutant
Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. PubMedA divIVA mutant has a moderate [(Pubmed)|26735940] to severe [(Pubmed)|11445541] sporulation defect. The protein
Catalyzed reaction/ biological activity
curvature sensitive membrane binding protein that recruits other proteins to the poles and the division septumDivIVA is required for polar localisation of MinC-MinD via MinJ. PubMedIt also recruits RacA to the distal pole of the prespore PubMed.DivIVA may anchor SpoIIE briefly to the assembling polar septum before SpoIIE is subsequently released into the forespore membrane and recaptured at the polar septum PubMedrequired for the compartment-specific activation of SigF PubMedactivates PrkC PubMedrequired for oriC placement during spore development PubMed Protein family
gpsB family (according to Swiss-Prot)Paralogous protein(s)
Domains
the first 60 amino acids constitute a conserved lipid binding domain. PubMedthe C-terminal domain is less conservedmultimerisation involves two coiled-coil motifs, one in the lipid binding domain, and the other one being present in the helical C-terminal domain PubMed PubMed Modification
phosphorylated on Arg-102 PubMedThe Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73 PubMedDivIVA from Streptococcus pneumoniae is phosphorylated at Threonine 201 by the Ser/Thr protein kinase Sktp1. PubMedPubMed Cofactors
not knownEffectors of protein activity
not knownStructure
Localization
DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles PubMed.forms rings at the division septum and patches at the cell poles PubMedmembrane targeting requires SecA PubMedassembles into a ring-like structure at the polar septum during sporulation PubMed Expression and Regulation
Biological materials
Mutant
Expression vector
DivIVA-Strep available herepGP1497 (N-terminal Strep-tag fused to C-terminus of divIVA, TEV-site, purification from E. coli, in pGP172), available in Jörg Stülke's lab GFP fusion
divIVA-gfp fusions available from the Hamoen Lab Two-hybrid system
FLAG-tag construct
Antibody
A polyclonal anti-DivIVA antiserum generated in rabbit is described here PubMed. Labs working on this gene/protein
References
Reviews
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Original Publications
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